引用本文请长按绿色字，点击复制：吕进微,林玉,冯耀宇,等.应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染[J].中山大学学报（医学科学版）,2026,47(01):11-16.
摘要：【目的】应用多种分子标记，评估其在鉴定广州市花都区花山镇野鼠粪便中的广州管圆线虫的有效性，并分析野鼠的感染状况。【方法】捕获广州花都区花山镇128只野鼠，采集野鼠粪便，采用FastDNA SPIN Kit for Soil试剂盒提取粪便总DNA，通过PCR扩增获得cytb、cox I和ITS2片段，PCR产物经测序鉴定，并对测序产物进行系统发育分析。【结果】通过PCR扩增和测序鉴定，仅有5份样本扩增出的cytb序列没有套峰，可以准确鉴定为广州管圆线虫。结果表明该地区褐家鼠的广州管圆线虫感染率为7.58%（5/66），而黄胸鼠、黄毛鼠、臭鼩鼱及板齿鼠中未检测到cytb序列，表明褐家鼠是广州管圆线虫的适宜宿主，且cytb序列适用于从野鼠粪便中鉴定广州管圆线虫的感染情况。【结论】Cytb序列是鉴定野鼠粪便中广州管圆线虫的良好分子标记，可以用于长期监测野鼠中的广州管圆线虫流行情况和危害潜力。
关键词：广州管圆线虫;cytb;野鼠;粪便;调查
Abstract：【Objective】Several molecular markers were evaluated for their reliability in detecting Angiostrongylus cantonensis in wild rodent feces and for determining the infection status of these rodents. 【Methods】A total of 128 wild rodents were captured in Huashan Town，Huadu district，Guangzhou. Their fecal samples were collected and total DNA was extracted with the FastDNA SPIN Kit for Soil. PCR amplification was performed to obtain cytb， cox I and ITS2 gene fragments， followed by Sanger sequencing and phylogenetical analysis. 【Results】Among all 128 fecal samples， only 5 had clean PCR bands and unambiguous cytb sequences suitable for reliable species identification. The infection rate of A. cantonensis was 7.58% （5/66） in Rattus norvegicus，whereas no cytb fragments were detected in Rattus tanezumi， Rattus losea， Suncus murinus and Bandicota indica. These data indicate that R. norvegicus is a highly suitable host for A. cantonensis， and the cytb gene serves as an effective molecular marker for detecting A. cantonensis in wild rodent feces.【Conclusion】The cytb is an effective molecular marker for detecting A. cantonensis in wild rodent feces， enabling long-term surveillance of its prevalence and potential public health threat.
Key words：Angiostrongylus cantonensis; cytb; wild rodent; feces; investigation
编 辑：武异洵
初 审：孙慧兰 黄子芸
复 审：余 菁
审核发布：高国全
